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                  • EI
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                  • 食品科學與工程領域高質量科技期刊分級目錄第一方陣T1
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                  中國精品科技期刊2020
                  李衛,房雷雷,張彥青,等. 桔梗多糖的復合酶提取、結構表征及抗氧化活性分析[J]. 食品工業科技,2023,44(18):283?291. doi: 10.13386/j.issn1002-0306.2022120100.
                  引用本文: 李衛,房雷雷,張彥青,等. 桔梗多糖的復合酶提取、結構表征及抗氧化活性分析[J]. 食品工業科技,2023,44(18):283?291. doi: 10.13386/j.issn1002-0306.2022120100.
                  LI Wei, FANG Leilei, ZHANG Yanqing, et al. Compound Enzyme Extraction of Platycodon grandiflorum Polysaccharides and Its Structure and Antioxidant Activity Characterization[J]. Science and Technology of Food Industry, 2023, 44(18): 283?291. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120100.
                  Citation: LI Wei, FANG Leilei, ZHANG Yanqing, et al. Compound Enzyme Extraction of Platycodon grandiflorum Polysaccharides and Its Structure and Antioxidant Activity Characterization[J]. Science and Technology of Food Industry, 2023, 44(18): 283?291. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022120100.

                  桔梗多糖的復合酶提取、結構表征及抗氧化活性分析

                  Compound Enzyme Extraction of Platycodon grandiflorum Polysaccharides and Its Structure and Antioxidant Activity Characterization

                  • 摘要: 目的:優化復合酶法提取桔梗多糖工藝,并初步分析桔梗多糖的結構和體外抗氧化活性。方法:分別考察酶添加量、料液比、酶解時間、酶解溫度對桔梗多糖提取率的影響,采用響應面法進行提取條件優化,利用高效液相色譜法(HPLC)測定多糖的分子量和單糖組成,應用核磁共振(NMR)和掃描電鏡(SEM)對多糖的糖苷鍵和形貌進行分析,并對多糖清除自由基的能力和還原力進行研究。結果:桔梗多糖的最佳提取工藝為纖維素酶、果膠酶、木瓜蛋白酶的添加量為2%,酶解時間90 min,料液比1:30 g/mL,酶解溫度50 ℃,在此條件下,多糖實際提取率為9.01%±0.07%,多糖含量達92%±0.76%。純化后得到桔梗多糖組分PGP-W-1,分子量為6.2 kDa,由甘露糖、鼠李糖、葡萄糖、半乳糖、木糖和阿拉伯糖按照摩爾比4.9:4.3:7.9:7.8:4.8:18.6組成,是一種具有α型糖苷鍵和β型糖苷鍵的吡喃型多糖。體外抗氧化試驗顯示PGP-W-1對DPPH自由基、ABTS+自由基和羥基自由基清除率IC50值分別為2.14、2.25、0.78 mg/mL。結論:本研究優選出的桔梗多糖提取工藝切實可行,多糖提取效率高并表現出良好的體外抗氧化活性。

                     

                    Abstract: Objective: Optimize the extraction process of Platycodon grandiflorum polysaccharides by compound enzyme method, and preliminarily analyze its structure and in vitro antioxidant activity. Methods: Response surface methodology was used to optimize the extraction conditions with the extraction rate of polysaccharides as the index and the addition amount of enzymes, solid-liquid ratio, enzymolysis time and enzymolysis temperature as the factors. The molecular weight and monosaccharide composition of purified polysaccharides were analyzed by high performance liquid chromatography (HPLC), the glycosidic bonds and surface morphology of purified polysaccharides were analyzed by nuclear magnetic resonance (NMR) and scanning electron microscopy (SEM), respectively, and the free radical scavenging ability and reducing power of purified polysaccharides were evaluated. Results: The optimum extraction conditions were as follows, the addition of cellulase, pectinase and papain was 2%, the enzymolysis time was 90 min, the solid-liquid ratio was 1:30 g/mL, the enzymolysis temperature was 50 ℃. Under these conditions, the actual extraction rate of polysaccharides was 9.01%±0.07%, and the purity of polysaccharides was 92%±0.76%. The purified polysaccharides component PGP-W-1 (6.2 kDa) was composed of mannose, rhamnose, glucose, galactose, xylose, and arabinose with a molar ratio of 4.9:4.3:7.9:7.8:4.8:18.6. NMR spectrum showed that PGP-W-1 was pyranose ring with α- and β-glycoside bond. The IC50 values of the PGP-W-1 on DPPH free radicals, ABTS+ free radicals and hydroxyl free radicals were 2.14, 2.25, and 0.78 mg/mL, respectively. Conclusion: The optimized extraction process of Platycodon grandiflorum polysaccharide was feasible with high extraction efficiency and showed excellent antioxidant activity in vitro.

                     

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