<track id="1517j"></track>

      <pre id="1517j"></pre>

          <ruby id="1517j"><ruby id="1517j"><b id="1517j"></b></ruby></ruby>
          <address id="1517j"></address>
          <track id="1517j"><strike id="1517j"><ol id="1517j"></ol></strike></track>

          <pre id="1517j"></pre>

          
          
          <track id="1517j"></track>
          <track id="1517j"></track>

          <track id="1517j"></track>

            <pre id="1517j"></pre><track id="1517j"></track>
                  • EI
                  • Scopus
                  • 食品科學與工程領域高質量科技期刊分級目錄第一方陣T1
                  • DOAJ
                  • EBSCO
                  • 北大核心期刊
                  • 中國核心學術期刊RCCSE
                  • JST China
                  • FSTA
                  • 中國精品科技期刊
                  • 中國農業核心期刊
                  • CA
                  • WJCI
                  • 中國科技核心期刊CSTPCD
                  • 中國生物醫學SinoMed
                  中國精品科技期刊2020
                  解小芬,胡光線,潘露,等. 黃精提取物改善D-半乳糖所致衰老小鼠器官功能及機制研究[J]. 食品工業科技,2023,44(18):449?457. doi: 10.13386/j.issn1002-0306.2022110220.
                  引用本文: 解小芬,胡光線,潘露,等. 黃精提取物改善D-半乳糖所致衰老小鼠器官功能及機制研究[J]. 食品工業科技,2023,44(18):449?457. doi: 10.13386/j.issn1002-0306.2022110220.
                  XIE Xiaofen, HU Guangxian, PAN Lu, et al. Effect of Polygonatum sibiricum Extract in Improving the Organ Function of D-Galactose-induced Aging Mice and Its Mechanism[J]. Science and Technology of Food Industry, 2023, 44(18): 449?457. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110220.
                  Citation: XIE Xiaofen, HU Guangxian, PAN Lu, et al. Effect of Polygonatum sibiricum Extract in Improving the Organ Function of D-Galactose-induced Aging Mice and Its Mechanism[J]. Science and Technology of Food Industry, 2023, 44(18): 449?457. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110220.

                  黃精提取物改善D-半乳糖所致衰老小鼠器官功能及機制研究

                  Effect of Polygonatum sibiricum Extract in Improving the Organ Function of D-Galactose-induced Aging Mice and Its Mechanism

                  • 摘要: 目的:旨在探討黃精提取物(Polygonatum sibiricum extract,PSE)對D -半乳糖(D-galactose,D -gal)致衰老小鼠重要臟器的保護作用及其潛在的分子機制。方法:將小鼠隨機分為五組(n=10):對照組、D-gal(500 mg/kg)組、PSE低(0.5 g/kg)、中(1 g/kg)、高(2 g/kg)劑量組。測定小鼠器官指數(胸腺、脾臟、肝臟、腎臟);測定血清中肌酸激酶(CK)、肌酸激酶同工酶(CK-MB)、谷丙轉氨酶(ALT)、谷草轉氨酶(AST)、堿性磷酸酶(ALP)、尿酸(UA)、尿素(UREA)、肌酐(CREA)、尿素肌酐比值(BUN/Scr)、腎小球濾過率(eGFR)活性;HE及Masson染色觀察各組小鼠皮膚、肝臟、腎臟、心臟、大腦、肺等器官病理學變化;用Real-time PCR法檢測各組小鼠肝臟、腎臟、心臟、大腦等組織中p53、p16、p21、RB、HO-1、Nrf2Keap1 mRNA的表達水平。結果:與D-gal組相比,PSE各劑量組胸腺、脾臟、肝臟和腎臟指數均升高(P<0.05);此外,在各治療組中,PSE降低了(P<0.05)小鼠血清中CK、CK-MB、ALT、AST、ALP、UA、UREA、CREA和BUN/Scr水平,升高了eGFR(P<0.05)水平;HE及Masson染色發現,PSE能減輕D-gal對小鼠重要器官造成的病理損傷;與對照組比較,模型組肝、腎、心、腦中p53、p16、p21、RB、Keap1 mRNA表達升高(P<0.05),HO-1、Nrf2 mRNA表達降低(P<0.05),與D-gal組比較,PSE各治療組小鼠肝臟、腎臟、心臟、大腦組織中p53、p16、p21、RB、Keap1 mRNA降低(P<0.05),HO-1、Nrf2 mRNA升高(P<0.05)。結論:PSE具有延緩衰老作用,其機制可能與其抑制p53/p21p16-RB通路以及Keap1/Nrf2/HO-1通路有關。

                     

                    Abstract: Objective: The purpose of this study was to explore the protective effects of Polygonatum sibiricum extract (PSE) on the important organs of D-galactose (D-gal)-induced aging mice and its potential molecular mechanisms. Methods: The mice were randomly divided into five groups (n=10): control group, D-gal (500 mg/kg) group, low-PSE dose (0.5 g/kg) group, medium-PSE dose (1 g/kg) group, and high-PSE dose (2 g/kg) group. The organ indices (thymus, spleen, liver, and kidney) were detected. The levels of creatine kinase (CK), creatine kinase isoenzyme (CK-MB), alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), uric acid (UA), urea (UREA), creatinine (CREA), urea–creatinine ratio (BUN/Scr), and glomerular filtration rate (eGFR) in serum were measured. Pathological changes in the skin, liver, kidney, heart, brain, and lung of mice in different groups were analyzed by HE and Masson staining. The expressions of p53, p16, p21, RB, HO-1, Nrf2, and Keap1 mRNA in the liver, kidney, heart, and brain of each group of mice were detected by real-time PCR. Results: The thymus, spleen, liver, and kidney indices were increased in each PSE treatment group compared with those in the D-gal group (P<0.05). Moreover, in each treatment group, PSE decreased (P<0.05) the levels of CK, CK-MB, ALT, AST, ALP, UA, UREA, CREA, and BUN/Scr in mouse serum and increased the level of eGFR (P<0.05). HE and Masson staining showed that PSE could reduce the pathological damage caused by D-gal to the vital organs of mice. Compared with the blank group, the model group presented increased expression levels of p53, p16, p21, RB, and Keap1 mRNA in the liver, kidney, heart, and brain (P<0.05) and decreased HO-1 and Nrf2 mRNA (P<0.05). Compared with the D-gal group, mice in each PSE treatment group exhibited decreased mRNA levels of p53, p16, p21, RB, and Keap1 in the liver, kidney, heart, and brain tissues (P<0.05) and increased mRNA levels of HO-1 and Nrf2 (P<0.05). Conclusion: PSE has anti-aging effects, and its mechanism may be related to its inhibition of the p53/p21, p16-RB, and Keap1/Nrf2/HO-1 pathways.

                     

                  /

                  返回文章
                  返回
                  亚洲一区精品黄