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                  • EI
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                  • 食品科學與工程領域高質量科技期刊分級目錄第一方陣T1
                  • DOAJ
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                  • 北大核心期刊
                  • 中國核心學術期刊RCCSE
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                  • 中國精品科技期刊
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                  • 中國生物醫學SinoMed
                  中國精品科技期刊2020
                  顧丹丹,侯靜宇,張金秀,等. 兩種方法制備的蛹蟲草多糖特性及體外生物活性對比分析[J]. 食品工業科技,2023,44(18):76?83. doi: 10.13386/j.issn1002-0306.2022110183.
                  引用本文: 顧丹丹,侯靜宇,張金秀,等. 兩種方法制備的蛹蟲草多糖特性及體外生物活性對比分析[J]. 食品工業科技,2023,44(18):76?83. doi: 10.13386/j.issn1002-0306.2022110183.
                  GU Dandan, HOU Jingyu, ZHANG Jinxiu, et al. Comparative Analysis of Characteristics and in Vitro Biological Activities of Cordyceps militaris Polysaccharide Prepared by Two Methods[J]. Science and Technology of Food Industry, 2023, 44(18): 76?83. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110183.
                  Citation: GU Dandan, HOU Jingyu, ZHANG Jinxiu, et al. Comparative Analysis of Characteristics and in Vitro Biological Activities of Cordyceps militaris Polysaccharide Prepared by Two Methods[J]. Science and Technology of Food Industry, 2023, 44(18): 76?83. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022110183.

                  兩種方法制備的蛹蟲草多糖特性及體外生物活性對比分析

                  Comparative Analysis of Characteristics and in Vitro Biological Activities of Cordyceps militaris Polysaccharide Prepared by Two Methods

                  • 摘要: 本研究以蛹蟲草發酵液為材料,采用殼聚糖絮凝法和水提醇沉法分別制備了絮凝多糖(XDT)和醇沉多糖(CDT),并對兩種方法制備所得多糖的得率、各組分含量、平均粒徑和復溶性進行了對比分析,利用掃描電鏡、傅里葉紅外光譜和剛果紅實驗對其微觀結構、官能團和空間構象進行表征,利用DPPH、超氧陰離子和羥自由基清除能力評價其抗氧化活性,以α-葡萄糖苷酶抑制率評價其降血糖活性,以HepG2細胞增殖抑制率評價其抗腫瘤活性。結果表明:殼聚糖絮凝法制備多糖得率為3.58%,是醇沉法的1.91倍;兩種多糖在掃描電鏡下的微觀結構存在差異,在紅外光譜下吸收圖譜相似,但XDT比CDT具有更多的三螺旋結構;XDT的平均粒徑更小,復溶速度更快;體外抗氧化實驗表明,在相同濃度下,對DPPH、超氧陰離子和羥自由基的清除能力,均表現為XDT>CDT;體外抑制α-葡萄糖苷酶活性實驗表明,低濃度時,兩種多糖的抑制率沒有顯著性差異,但隨著濃度增加,在相同條件下,XDT的抑制活性明顯強于CDT;體外抑制腫瘤細胞增殖能力實驗表明,在相同處理濃度下,XDT對HepG2細胞的增殖抑制作用優于CDT。綜上,殼聚糖絮凝法制備的蛹蟲草多糖得率更高、平均粒徑更小、復溶性更佳,XDT具備較強的體外抗氧化、降血糖和抗腫瘤活性。

                     

                    Abstract: Cordyceps militaris polysaccharides from the fermentation broth were prepared via chitosan flocculation and water extraction and alcohol precipitation methods to obtain the flocculated polysaccharide (XDT) and alcohol precipitation polysaccharide (CDT) respectively. The extraction yields, component content, average particle sizes and solubility of XDT and CDT were comparatively investigated. The microstructures, functional groups and spatial conformations were characterized by scanning electron microscopy, Fourier infrared spectroscopy and Congo red assay. The antioxidant activity was evaluated by scavenging ability of DPPH, superoxide anion and hydroxyl radical. The hypoglycemic activity was estimated by α-glucosidase inhibitory ability, and the antitumor cell activity was analyzed by inhibition of HepG2 cell proliferation. The results showed a yield of 3.58% for XDT, 1.91 times that of CDT. CDT and XDT exhibited different microstructures under electron microscopy, and similar absorption patterns under infrared spectrum, while XDT contained more triple helix structures than CDT. XDT displayed smaller average particle sizes but a faster dissolution rate than CDT. In vitro antioxidant tests showed that XDT exhibited significantly higher scavenging ability of DPPH, superoxide anion and hydroxyl radical than CDT at the same concentration. There was no significant difference between the XDT and CDT in the inhibition of α-glucosidase at low concentration, but XDT showed significantly higher inhibition than CDT as the increase of concentration. XDT was found to be a stronger inhibitor of HepG2 cells proliferation than CDT at the same treatment concentration. In summary, XDT showed higher yields, smaller average particle sizes, faster solubility, and higher antioxidant, hypoglycemic, and antitumor activity in vitro.

                     

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