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                  • EI
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                  • 食品科學與工程領域高質量科技期刊分級目錄第一方陣T1
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                  中國精品科技期刊2020
                  劉旺,白金波,張王娟,等. 黃精多糖理化性質及其對D-半乳糖誘導小鼠氧化損傷的保護作用[J]. 食品工業科技,2023,44(18):425?433. doi: 10.13386/j.issn1002-0306.2022100250.
                  引用本文: 劉旺,白金波,張王娟,等. 黃精多糖理化性質及其對D-半乳糖誘導小鼠氧化損傷的保護作用[J]. 食品工業科技,2023,44(18):425?433. doi: 10.13386/j.issn1002-0306.2022100250.
                  LIU Wang, BAI Jinbo, ZHANG Wangjuan, et al. Study on the Physicochemical Properties of Polysaccharide from Polygonatum sibiricum and Its Protective Effect on D-Galactose-Induced Oxidative Damage in Mice[J]. Science and Technology of Food Industry, 2023, 44(18): 425?433. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100250.
                  Citation: LIU Wang, BAI Jinbo, ZHANG Wangjuan, et al. Study on the Physicochemical Properties of Polysaccharide from Polygonatum sibiricum and Its Protective Effect on D-Galactose-Induced Oxidative Damage in Mice[J]. Science and Technology of Food Industry, 2023, 44(18): 425?433. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100250.

                  黃精多糖理化性質及其對D-半乳糖誘導小鼠氧化損傷的保護作用

                  Study on the Physicochemical Properties of Polysaccharide from Polygonatum sibiricum and Its Protective Effect on D-Galactose-Induced Oxidative Damage in Mice

                  • 摘要: 本研究從黃精根莖部位提取、分離純化一種均一多糖組分,分析其理化性質和對氧化損傷的保護作用,并探討其潛在的保護機制。采用水提醇沉、DEAE-纖維素陰離子交換柱與Sephadex G-100凝膠層析柱從黃精根莖提取、分離得到均一多糖組分PSP。采用苯酚硫酸法、間羥基聯苯法、高效凝膠滲透色譜和傅里葉紅外光譜等分析PSP的理化性質,并通過D-半乳糖誘導氧化損傷小鼠模型探究PSP的體內抗氧化作用。結果表明,PSP的碳水化合物含量為94.42%±14.73%,其分子量為5566.41 Da,含有β型的呋喃環或吡喃環果糖,以及較長的支鏈和較多的分支。與模型組相比,PSP高劑量組血清、腦組織、肝臟組織的SOD、GSH-Px與T-AOC水平顯著升高(P<0.05),MDA水平顯著降低(P<0.05);HE染色發現PSP處理組的肝臟損傷明顯得到改善,且肝臟中Nrf2和HO-1的表達顯著增強(P<0.05)。因此,結果表明黃精多糖PSP可能通過Nrf2/HO-1信號通路保護小鼠的氧化損傷。

                     

                    Abstract: In this study, a homogeneous polysaccharide fraction was extracted and purified from the rhizome of Polygonatum sibiricum, its physicochemical properties and protective effect against oxidative damage were analyzed, and its potential protective mechanism was initially explored. The homogeneous polysaccharide (PSP) was obtained by water extraction, alcohol precipitation, and separation on DEAE-52 cellulose and Sephadex G-100 columns. The phenol sulfate method, m-hydroxybiphenyl method, high performance gel permeation chromatography and Fourier infrared spectroscopy were used to analyze the physicochemical properties of PSP. The in vivo antioxidant effects of PSP were investigated in a mouse model of D-galactose induced oxidative damage. The results showed that the carbohydrate content of PSP was 94.42%±14.73% and its molecular weight was 5566.41 Da. PSP was also found to contain β-type furan or pyran fructose, as well as longer branched chains with more branches. Compared with the model group, the levels of SOD, GSH-Px and T-AOC significantly increased (P<0.05), and MDA levels significantly decreased (P<0.05) in serum, brain tissue and liver tissue of the PSP high dose groups, HE staining revealed that liver damage was significantly ameliorated and the expression of Nrf2 and HO-1 protein in the liver was significantly enhanced in the PSP-treated group (P<0.05). It could be concluded that PSP had a protective effect on oxidative damage in mice, and its protective mechanism might be related to the Nrf2/HO-1 signaling pathway.

                     

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