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                  • EI
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                  • 食品科學與工程領域高質量科技期刊分級目錄第一方陣T1
                  • DOAJ
                  • EBSCO
                  • 北大核心期刊
                  • 中國核心學術期刊RCCSE
                  • JST China
                  • FSTA
                  • 中國精品科技期刊
                  • 中國農業核心期刊
                  • CA
                  • WJCI
                  • 中國科技核心期刊CSTPCD
                  • 中國生物醫學SinoMed
                  中國精品科技期刊2020
                  姜聰一,康瑞,于濤,等. 苯乳酸對單增李斯特菌生物被膜的影響[J]. 食品工業科技,2023,44(18):164?172. doi: 10.13386/j.issn1002-0306.2022100239.
                  引用本文: 姜聰一,康瑞,于濤,等. 苯乳酸對單增李斯特菌生物被膜的影響[J]. 食品工業科技,2023,44(18):164?172. doi: 10.13386/j.issn1002-0306.2022100239.
                  JIANG Congyi, KANG Rui, YU Tao, et al. Effects of Phenyllactic Acid on Listeria monocytogenes Biofilms[J]. Science and Technology of Food Industry, 2023, 44(18): 164?172. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100239.
                  Citation: JIANG Congyi, KANG Rui, YU Tao, et al. Effects of Phenyllactic Acid on Listeria monocytogenes Biofilms[J]. Science and Technology of Food Industry, 2023, 44(18): 164?172. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100239.

                  苯乳酸對單增李斯特菌生物被膜的影響

                  Effects of Phenyllactic Acid on Listeria monocytogenes Biofilms

                  • 摘要: 為研究苯乳酸(Phenyllactic Acid,PLA)對單增李斯特菌(Listeria monocytogenes,Lm)生物被膜的影響,采用結晶紫法檢測Lm生物被膜生物量、倒置顯微鏡和掃描電鏡觀察Lm生物被膜結構;分別采用苯酚硫酸法、福林酚法和二苯胺法測定生物被膜中胞外多糖、胞外蛋白和胞外DNA的含量;利用實時熒光定量PCR檢測PLA對Lm生物被膜形成相關基因轉錄水平的影響。結果表明:PLA對8株Lm菌株的最小抑菌濃度值均為6 mg/mL。PLA通過抑制胞外多糖和胞外蛋白的合成抑制Lm生物被膜的形成。對于Lm成熟生物被膜,PLA通過減少胞外多糖和胞外蛋白的量破壞生物被膜結構、降低生物被膜生物量。PLA還能抑制Lm的運動性。在PLA作用下Lm群體感應系統agr的轉錄水平顯著(P<0.05)降低。作為一種天然抗菌物質,PLA在控制Lm及其生物被膜對食品污染方面具有潛在的應用價值。

                     

                    Abstract: To investigate the effect of phenyllactic acid (PLA) on Listeria monocytogenes biofilms, crystal violet method was used to measure the biomass of L. monocytogenes biofilms, and inverted microscope and scanning electron microscope were used to observe the structure of L. monocytogenes biofilms, the content of extracellular polysaccharide, extracellular protein and extracellular DNA in biofilm was measured by phenol-sulfuric acid method, Folin-phenol method and diphenylamine method, respectively. The effect of PLA on the transcription of genes associated with biofilm formation of L. monocytogenes was detected by real-time quantitative PCR. The results showed that the minimum inhibitory concentration values of PLA against eight L. monocytogenes strains were 6 mg/mL. PLA inhibited L. monocytogenes biofilm formation by reducing the synthesis of extracellular polysaccharides and extracellular proteins. For mature biofilms of L. monocytogenes, PLA destroyed the biofilm structure and reduced the biofilm biomass by reducing the amount of extracellular polysaccharides and extracellular proteins. PLA also inhibited the swarming motility of L. monocytogenes. The transcription levels of quorum sensing system agr in L. monocytogenes were significantly (P<0.05) decreased in the presence of PLA. As a natural antimicrobial compound, PLA had the potential application to control food contamination by L. monocytogenes and its biofilms.

                     

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