<track id="1517j"></track>

      <pre id="1517j"></pre>

          <ruby id="1517j"><ruby id="1517j"><b id="1517j"></b></ruby></ruby>
          <address id="1517j"></address>
          <track id="1517j"><strike id="1517j"><ol id="1517j"></ol></strike></track>

          <pre id="1517j"></pre>

          
          
          <track id="1517j"></track>
          <track id="1517j"></track>

          <track id="1517j"></track>

            <pre id="1517j"></pre><track id="1517j"></track>
                  • EI
                  • Scopus
                  • 食品科學與工程領域高質量科技期刊分級目錄第一方陣T1
                  • DOAJ
                  • EBSCO
                  • 北大核心期刊
                  • 中國核心學術期刊RCCSE
                  • JST China
                  • FSTA
                  • 中國精品科技期刊
                  • 中國農業核心期刊
                  • CA
                  • WJCI
                  • 中國科技核心期刊CSTPCD
                  • 中國生物醫學SinoMed
                  中國精品科技期刊2020
                  谷悅,唐會鑫,李朔,等. QuEChERS-超高效液相色譜-三重四極桿串聯質譜法測定水果制品和肉醬中10種四環素類抗生素[J]. 食品工業科技,2023,44(18):313?320. doi: 10.13386/j.issn1002-0306.2022100072.
                  引用本文: 谷悅,唐會鑫,李朔,等. QuEChERS-超高效液相色譜-三重四極桿串聯質譜法測定水果制品和肉醬中10種四環素類抗生素[J]. 食品工業科技,2023,44(18):313?320. doi: 10.13386/j.issn1002-0306.2022100072.
                  GU Yue, TANG Huixin, LI Shuo, et al. Determination of 10 Tetracycline Antibiotics in Fruit Products and Meat Sauce by QuEChERS with Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2023, 44(18): 313?320. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100072.
                  Citation: GU Yue, TANG Huixin, LI Shuo, et al. Determination of 10 Tetracycline Antibiotics in Fruit Products and Meat Sauce by QuEChERS with Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry[J]. Science and Technology of Food Industry, 2023, 44(18): 313?320. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022100072.

                  QuEChERS-超高效液相色譜-三重四極桿串聯質譜法測定水果制品和肉醬中10種四環素類抗生素

                  Determination of 10 Tetracycline Antibiotics in Fruit Products and Meat Sauce by QuEChERS with Ultra Performance Liquid Chromatography-Triple Quadrupole Tandem Mass Spectrometry

                  • 摘要: 使用QuEChERS結合超高效液相色譜-三重四極桿串聯質譜(Ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry,UPLC-MS/MS),建立檢測水果制品和肉醬中10種四環素類抗生素的分析方法。通過優化質譜參數、色譜條件和前處理技術,確定了最佳實驗條件。樣品經0.2%甲酸乙腈超聲提取后,水果制品和肉醬分別用N-丙基乙二胺(PSA)和C18凈化,經ZORBAX SB-Aq色譜柱(2.1 mm×150 mm,3.5 μm)分離,UPLC-MS/MS測定。結果顯示,在各自線性范圍內,10種四環素類抗生素具有良好的線性關系,相關系數(r)大于0.999;方法的檢出限(LOD,S/N=3)為0.09~3.92 μg/kg,定量限(LOQ,S/N=10)為0.31~13.05 μg/kg;在75、300和750 μg/kg 3個不同加標水平下的平均回收率為70.0%~101.0%,相對標準偏差(Relative standard deviation,RSD)為1.0%~10.3%(n=7)。該方法靈敏、簡便,適用于水果制品和肉醬中10種四環素類抗生素的快速同步檢測。

                     

                    Abstract: A method for the determination of 10 tetracycline antibiotics in fruit products and meat sauce was established by QuEChERS coupled with ultra performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS). The optimal experimental conditions were determined by optimizing the mass spectrometric parameters, chromatographic conditions and pre-treatment techniques. The samples were extracted by 0.2% formic acid acetonitrile under ultrasonication, and then purified with PSA for fruit products and C18 for meat sauce. The target analytes were separated on a ZORBAX SB-Aq column (2.1 mm×150 mm, 3.5 μm), and detected by UPLC-MS/MS. The results demonstrated that the 10 tetracycline antibiotics showed good linearity in the respective linear range with the correlation coefficients (r) higher than 0.999. The limits of detection (S/N=3) were 0.09~3.92 μg/kg, and the limits of quantification (S/N=10) were 0.31~13.05 μg/kg. The average recoveries at three different spiked levels of 75, 300 and 750 μg/kg ranged from 70.0% to 101.0% with the relative standard deviation (RSD) of 1.0%~10.3% (n=7). The method is sensitive, simple and suitable for the rapid and simultaneous detection of 10 tetracycline antibiotics in fruit products and meat sauce.

                     

                  /

                  返回文章
                  返回
                  亚洲一区精品黄