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                  • EI
                  • Scopus
                  • 食品科學與工程領域高質量科技期刊分級目錄第一方陣T1
                  • DOAJ
                  • EBSCO
                  • 北大核心期刊
                  • 中國核心學術期刊RCCSE
                  • JST China
                  • FSTA
                  • 中國精品科技期刊
                  • 中國農業核心期刊
                  • CA
                  • WJCI
                  • 中國科技核心期刊CSTPCD
                  • 中國生物醫學SinoMed
                  中國精品科技期刊2020
                  張志毅,白若熙,宗愛珍,等. 菊芋中抗氧化成分的在線鑒定和構效關系分析[J]. 食品工業科技,2023,44(18):307?312. doi: 10.13386/j.issn1002-0306.2022080031.
                  引用本文: 張志毅,白若熙,宗愛珍,等. 菊芋中抗氧化成分的在線鑒定和構效關系分析[J]. 食品工業科技,2023,44(18):307?312. doi: 10.13386/j.issn1002-0306.2022080031.
                  ZHANG Zhiyi, BAI Ruoxi, ZONG Aizhen, et al. Online Identification and Constitutive Relationship Analysis of Antioxidant Components in Jerusalem Artichoke[J]. Science and Technology of Food Industry, 2023, 44(18): 307?312. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080031.
                  Citation: ZHANG Zhiyi, BAI Ruoxi, ZONG Aizhen, et al. Online Identification and Constitutive Relationship Analysis of Antioxidant Components in Jerusalem Artichoke[J]. Science and Technology of Food Industry, 2023, 44(18): 307?312. (in Chinese with English abstract). doi: 10.13386/j.issn1002-0306.2022080031.

                  菊芋中抗氧化成分的在線鑒定和構效關系分析

                  Online Identification and Constitutive Relationship Analysis of Antioxidant Components in Jerusalem Artichoke

                  • 摘要: 本研究以高效液相色譜-飛行時間質譜聯用結合清除1,1-二苯基-2-三硝基苯肼(DPPH)的在線檢測技術,建立快速篩選菊芋中咖啡??鼘幩犷惓煞值姆椒?,并對其抗氧化活性進行評價。以乙腈-水(0.1%甲酸)為流動相進行梯度洗脫,經C18柱分離后,待測樣品進入PEEK盤管與DPPH自由基溶液混合反應后進入紫外檢測器,采用280和517 nm檢測,并通過517 nm產生的負峰實現咖啡??鼘幩犷惓煞值脑诰€篩選。結果表明,在負離子模式下根據精確分子量結合標準品對照,篩選出菊芋中的5種抗氧化成分:綠原酸、咖啡酸、3,4-O-二咖啡??鼘幩?、3,5-O-二咖啡??鼘幩?、4,5-O-二咖啡??鼘幩?。各組分對DPPH自由基、ABTS+自由基、超氧陰離子自由基清除率分別在54.63%~95.43%、8.32%~69.63%、27.37%~51.40%范圍內,并均與濃度呈正相關。本研究快速鑒定了菊芋中咖啡??鼘幩犷惓煞植⑻骄科淇寡趸钚?,為菊芋中咖啡??鼘幩犷惢衔锏拈_發利用提供了科學依據。

                     

                    Abstract: In this study, a new method based on the HPLC online scavenging diphenyl-1-picrylhydrazyl (DPPH) radical activity and Q-TOF/MS for the rapid screening and identification of caffeoylquinic acids in Jerusalem artichoke, and its antioxidant activity was evaluated. The mobile phase was acetonitrile-0.1% formic acid aqueous solution (gradient elution), and the samples were separated by C18 column. The samples were mixed with DPPH radical solution in PEEK tube and then entered UV detector, the detection wavelength were 280 and 517 nm, and the online screening of caffeoylquinic acid components was realized by the negative peak generated at 517 nm. Results showed that, under the negative ion mode, according to the precise molecular weight combined with the standards, five antioxidant compounds were screened from the Jerusalem artichoke, including chlorogenic acid, caffeic acid, 3,4-O-dicaffeoylquinic acid, 3,5-O-dicaffeoylquinic acid, 4,5-O-dicaffeoylquinic acid. The free radical scavenging rate of each component to DPPH, ABTS+ and superoxide anion was in the range of 54.63%~95.43%, 8.32%~69.63%, 27.37%~51.40%, and were positively correlated with the concentration. In this study, the caffeoylquinic acids in Jerusalem artichoke were rapidly identified and their antioxidant activities were explored, which provided a scientific basis for the development and utilization of caffeoylquinic acids in Jerusalem artichoke.

                     

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